ABSTRACT
As the essential characteristics of malignant cancer,invasion and metastasis are the main reason for failure treatment and high death rate of cancer patients.It is necessary to find out safe and effective anti-invasion and anti-metastasis drugs for the improvement of clinical efficacy.With the definite therapeutic effect and mild side-effects,Chinese materia medica(CMM) including curcumin,peiminine,β-elemi and so on,have become hotspots in anti-invasion and anti-metastasis drugs research.The mechanisms for the effective ingredients of CMM anti-tumor invasion and metastasis were reviewed in this paper.
ABSTRACT
AIM:To investigate the effects of luteolin on invasion,migration and adhesion of human hepatocelluar carcinoma HepG2 cells.METHODS:HepG2 cells were cultured and treated with luteolin at 10,20 and 40 μmol/L respectively.The invasion capability was examined by cell invasion assay.The migration ability was examined by wound healing assay.The adhesion capability was measured by adhesion assay.The protein levels of E-cadherin,N-cadherin,vimentin and Snai1 were determined by Western blot analysis.RESULTS:Luteolin inhibited the invasion,migration and adhesion ability of HepG2 cells in vitro in a dose-dependent manner.After treatment with luteolin,the expression of E-cadherin was increased significantly and the expression of N-cadherin,vimentin and Snai1 were decreased significantly.CONCLUSION:Luteolin inhibits the invasion,migration and adhesion ability of human hepatocelluar carcinoma HepG2 cells.The mechanism may be related to the regulatory effects of luteolin on epithelial-mesenchymal transition.
ABSTRACT
As the essential characteristics of malignant cancer,invasion and metastasis are the main reason for failure treatment and high death rate of cancer patients.It is necessary to find out safe and effective anti-invasion and anti-metastasis drugs for the improvement of clinical efficacy.With the definite therapeutic effect and mild side-effects,Chinese materia medica(CMM) including curcumin,peiminine,β-elemi and so on,have become hotspots in anti-invasion and anti-metastasis drugs research.The mechanisms for the effective ingredients of CMM anti-tumor invasion and metastasis were reviewed in this paper.
ABSTRACT
AIM:To investigate the effects of luteolin on invasion,migration and adhesion of human hepatocelluar carcinoma HepG2 cells.METHODS:HepG2 cells were cultured and treated with luteolin at 10,20 and 40 μmol/L respectively.The invasion capability was examined by cell invasion assay.The migration ability was examined by wound healing assay.The adhesion capability was measured by adhesion assay.The protein levels of E-cadherin,N-cadherin,vimentin and Snai1 were determined by Western blot analysis.RESULTS:Luteolin inhibited the invasion,migration and adhesion ability of HepG2 cells in vitro in a dose-dependent manner.After treatment with luteolin,the expression of E-cadherin was increased significantly and the expression of N-cadherin,vimentin and Snai1 were decreased significantly.CONCLUSION:Luteolin inhibits the invasion,migration and adhesion ability of human hepatocelluar carcinoma HepG2 cells.The mechanism may be related to the regulatory effects of luteolin on epithelial-mesenchymal transition.